Due to the irreversible adsorption of cellulase on specific fabrics, it accelerates the re deposition of bright blue dyes onto cellulose fibers. Therefore, the presence of cellulase is the main reason for denim washing and dyeing. This research conclusion mainly considers the interaction between specific fibers and cellulase proteins: if there are many proteins bound by fibers, the degree of retrogradation is deep; If there is less protein bound to the fibers, the problem of re staining is shallow. In fact, the interaction between different types of cellulases and indigo varies. Whether the enzyme protein binds to the fiber determines the interaction between the cellulase protein and the brilliant blue, thereby affecting the effect of staining.

Theoretically, when cellulase acts on cellulose fibers, the first thing is the chemical binding between the enzyme and the fiber. Due to the irreversible attachment between the enzyme and the fiber, some enzyme proteins will not be desorbed from the fiber, which intensifies the combination of dyes and fibers. Moreover, due to the existence of the chemical bond between the dye and the enzyme protein, the enzyme protein becomes a solid bridge between dyes and fibers. This combination is difficult to remove using ordinary cleaning methods, so the fermentation washing process is the most important post finishing process for producing bright blue dye in denim clothing.

From the perspective of enzyme species, most of the liquid enzymes used in the garment washing process are acidic cellulases, while most of the solid enzymes are neutral cellulases. Acidic enzymes contain many polar structures that bind to cellulose fibers, so they can form strong chemical bonds with cotton fibers; Most neutral enzymes undergo genetic modification to reduce these polar structures, so the degree of infection resistance of neutral enzymes is much lower than that of acidic enzymes.

From the perspective of fabric types, the staining of cotton fibers is more difficult to remove than that of other chemical fibers, which can also be explained theoretically. Cellulase only acts on cotton fibers and has a weak binding force with other fibers, making it easy to clean them off through the action of surfactants. Therefore, cotton fabrics usually have more severe staining.

Other conditions are more or less due to the influence on the binding between blue dye, cellulase, and fiber, thereby affecting the degree of dyeing.